NGS Hands On Workshop

Many people say Next Gen Sequencing is the revolutionary change in sequencing methodologies!! Since we at Genotypic believe that the best way to understand the full implications of a technology is through spreading the knowhow and applying it across multiple platforms, we conducted an extensive hands on workshop on Next Generation Sequencing (NGS) in Jan 2011. The six day workshop was planned as an interactive programme with experts some interesting tips and tricks around the effective use of the technology.

The first day was spent understanding the best and most efficient ways to do library prep. On the second day the prepared microbial libraries were put to test on the Illumina GAIIx on a 54 base paired end run while other participants moved on to perform exome capture of Human samples using the Agilent sureselect platform with the helpful guidance of Henner Freidle, a Senior APS from Agilent, Germany. The evening saw very useful presentations by representatives from Agilent, Illumina and Genotypic. The next two days were hectic with the participants learning about designing of baits for array capture, validation of capture, sequencing and qPCR setups.

The fifth day had a very interactive quiz session on the learnings of the entire workshop. We celebrated the end of the sequencing run during the workshop with a fine Chinese lunch.On the last day, the participants got an opportunity to do sequence analysis of their own samples that were sequenced during the workshop. A centrifuge sponsored by Genereach Biotech was given to the top performer among the participants.

A few testimonials from the workshop participants:

“Well Done, Good Team Work”…

“Congratulations and thanks for conducting such a nice workshop”…

“I learned a lot in the wet lab”…

” It is great to see such an excellent workshop organization, both in general and in detail”…

www.nextgenseq.com/workshop

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