NGS Sequencing and Analysis Facility at Genotypic
From Library Preparation to Indepth Data Analysis

Coming up: Cancer workshop highlighting 'Next Generation Sequencing in clinics'

  • Home
  • Lab
  • Ion Torrent
  • GAIIx
  • Primary Analysis
  • Secondary Analysis
  • Custom Analysis
  • NGS Validation
  • qPCR

We are very pleased to announce the new arrivals at Genotypic-
ION Torrent and 2nd ILLUMINA GAIIx

We specialize in long (>140 bases) paired end reads

Short turn around time for short reads

Second ILLUMINA GAIIx

ION TORRENT

GAIIx
iontorrent

ION Torrent Sequencing
12

 

Download - NGS/Microarray Applications Brochure

(to download , right click and select "Save As")

lab

NGS Lab

Pre-sequencing steps done at state-of-the-art molecular biology lab Library Preparation for:
NGS/ Illumina/ Solexa GAIIX, HiSeq-for Singleton, Paired End, Mate Pair, Targeted Re-sequencing (SureSelect -In-solution and On-Array Capture), genomic DNA prep, mRNA/Transcriptome sequencing, SAGE, Digital Gene Expression, small RNA/ micro RNA sequencing, ChIP sequencing, Methylome/ Bisulfite Sequencing.
 
lib_prep

     
     
     

We are very pleased to inform you the arrival of Ion torrent at Genotypic. The Ion Torrent is simpler, cost effective, and more scalable than any other sequencing technology. The Ion Torrent sequencer is a benchtop system utilizing ground-breaking and disruptive semiconductor technology that enables rapid and scalable sequencing experiments.

iontorrent

Ion Torrent


Technology
Ion Torrent technology uses a massively parallel array of proprietary semiconductor sensors to perform direct real-time measurement of the hydrogen ions produced during DNA replication. A high-density array of wells on the Ion semiconductor chips provides millions of individual reactors, and integrated fluidics allow reagents to flow over the sensor array. This unique combination of fluidics, micro-machining, and semiconductor technology enables the direct translation of genetic information (DNA) to digital information (DNA sequence), rapidly generating large quantities of high-quality data.

  • Direct, real-time detection technology
  • Simplest sequencing chemistry: hydrogen ion detection (pH meter)
  • Short run time supports fast research cycle and faster time to publication

Applications
Amplicon sequencing, microbial sequencing, RNA-Seq, ChIP-Seq, targeted resequencing, copy number analysis, methylation, paired-ends

If you have performed sequencing runs with illumina and want to have longer reads to close gaps / reconfirm data, Ion torrent is the solution for you. 

ION Torrent Sequencing

12

 

 

GAIIx

Illumina GAIIx

Sequencing by Synthesis - Reverse Dye Terminator Chemistry

 
seq

PE

Paired End (PE) Module

GAIIx supported with precise microfluidics for paired end runs

 

seq
     

primary analysis

Primary Analysis

Real time moniroting of sequencing runs

 

primary

seqqc

Simple Desktop Application for Rapid Quality Control and Reporting of NGS Data and Processed Sequence Files. Highend Server side application is available for download

 
seqqc
     

pipelineserver

Illumina Pipeline Server

Raw data processed through Illumina pipeline software

  • Data generated from GAIIx is transferred to the server on the run

  • High end server capable of handling analysis of the previous run and transferring of data from the current run simultaneously.

  • Pipeline Analysis - Alignment and Variant Analysis (CASAVA)

 

 


secondary
     

ngsdataanalysislab

NGS Data Analysis Team

Making sense of the ATGCs:
QC, Alignment, Analysis to Biological Interpretation

 

Custom
     

validation

pic

Agilent Stratagene MX3005P

Quantitative and Semi-quantitative validations services are offered from Genotypic using Taqman and SYBR Green assays. RT-PCR is a very sensitive and quantitave tool for validations. Genotypic covers all aspects of the project from assay design, to nucleic acid isolation, to data analysis

Specilizations

qPCR Services
DNA Validations
qRTPCR Services
Microarrays Validation
  • Primer Designing
  • Primer Validation by BioAnalyzer
  • Fold Change calculations -Raw Ct values, ??Ct Analysis
  • Data Analysis
  • Report Generation
  •  Methylations 
  • ChIP On chip 
  • SNP Analysis 
  • Gene Expression studies

Create primer designs to suit your study or choose from our extensive and ready to order catalog - Human, Aarabidopsis, House keeping genes, Apoptosis and many more for RNA and gDNA are available. To know more email us at genomics@genotypic.co.in

 

 


agilent sap